abstract：:The chemicals 6-mercaptopurine (6-MP) and methotrexate (MTX) are the cornerstones in the maintenance treatment of acute lymphoblastic leukemia. The intracellular metabolism of 6-MP to 6-thioguanosine nucleotides (TGN) via 6-thioinosine 5'-monophosphate (TIMP) is crucial for its cytotoxic effect. MTX inhibits purine de...

journal_title：Leukemia research

authors： Liliemark J,Pettersson B,Peterson C

更新日期：1992-01-01 00:00:00

abstract：:Methods for ex vivo purging of neoplastic cells from harvested marrow are being developed to increase the efficacy of autologous transplantation. One approach is selective photosensitization, using sensitizing compounds and light radiation. Pyrene-containing fatty acids and lipids are potent photosensitizers, e.g. 12-...

journal_title：Leukemia research

authors： Fibach E,Rabia SA,Gatt S

更新日期：1992-01-01 00:00:00

abstract：:We report on four cases of trisomy 14 as the sole anomaly. Three cases were myelodysplastic syndromes and one was a non-Hodgkin's lymphoma. This anomaly is mainly in myeloid disorders and still remains to be well documented. On the other hand, we show this anomaly to be also a non-random anomaly in lymphoproliferative...

journal_title：Leukemia research

authors： Brizard A,Guilhot F,Babin P,Burucoa C,Tanzer J,Huret JL

更新日期：1992-01-01 00:00:00

abstract：:The Philadelphia (Ph1) chromosome is a specific structural abnormality in which the abl oncogene is activated due to the formation of the novel chimeric gene, bcr/abl. To investigate the clinicopathological role of bcr/abl in Ph1-positive chronic myelogenous leukaemia (CML), we studied the clonal origin of haematopoie...

journal_title：Leukemia research

authors： Sadamura S,Umemura T,Takahira H,Hirata J,Nishimura J,Nawata H

更新日期：1992-01-01 00:00:00

abstract：:Information about the expression of some myelomonocytic markers in lymphocytes of patients with B-CLL is scarce. We studied the CD13, CD14, CD11c and CD68 surface antigens in 42 controls and in 38 patients with B-CLL to detect their possible reactivity. Eighty-nine percent of B-CLL expressed very strongly the CD14 ant...

journal_title：Leukemia research

authors： Woessner S,Florensa L,Besses C,Vilà RM,Gallés C,Palou L,Sabrafen JS

更新日期：1992-01-01 00:00:00

abstract：:In order to investigate the clinical significance of IgG subclass levels in adult patients treated for acute lymphocytic (ALL) or myelogenous leukemias (AML), patients were tested before and in regular intervals during and after chemotherapy. Ten patients treated for acute lymphocytic leukemia had normal total IgG and...

journal_title：Leukemia research

authors： Gause A,Schmitz G,von Kalle AK,Lathan B,Diehl V,Pfreundschuh M

更新日期：1992-01-01 00:00:00

abstract：:The efficiency of gene transfer into human leukemia cell lines by electroporation was investigated. For both transient expression (beta-galactosidase gene) and stable transformation (neomycin resistance gene), the transfer efficiency into leukemia cell lines using a square wave pulse was superior to that using an expo...

journal_title：Leukemia research

authors： Takahashi M,Furukawa T,Saitoh H,Aoki A,Koike T,Moriyama Y,Shibata A

更新日期：1991-01-01 00:00:00

abstract：:We report the characterization of a novel human T-cell line, HPB-MLp-W, which was established from blastic cells of a lymph node specimen from a patient with non-Hodgkin's lymphoma. They demonstrated the T-cell association antigens, CD2 and CD4, but no CD3, CD8, CD1, CD5, CD7 nor T-cell antigen receptor on their cell ...

journal_title：Leukemia research

authors： Morikawa S,Morikawa K,Hara J,Nagasaki M,Nakano A,Oseko F

更新日期：1991-01-01 00:00:00

abstract：:Leukemia-prone AKR mice were exposed twice a week to a 6 mT, 12 Hz or 460 Hz pulsed magnetic field for 30 min. If we take into account the five consecutive generations of mice, the above exposure actually took place in utero and, or during their life span. There was no difference in the incidence of leukemia or in the...

journal_title：Leukemia research

authors： Bellossi A

更新日期：1991-01-01 00:00:00

abstract：:One of the major limitations of DNA flow cytometry (FCM) in hematologic malignancies is the lack of information about the proliferation activity of subpopulations of the heterogeneous bone marrow (BM) compartment. We studied the S-phase DNA content of immunophenotypically defined BM subpopulations (CD2+; CD19+; CD2/CD...

journal_title：Leukemia research

authors： Brons P,Pennings A,Haanen C,Wessels H,Boezeman J

更新日期：1991-01-01 00:00:00

abstract：:We have analysed the configuration of immunoassociated genes and the karyotypes of 30 patients with acute myelocytic leukemia (AML) and 10 with chronic myelocytic leukemia in blast crisis (CML-BC). In AML, the frequencies of T-cell receptor (TcR) beta, gamma, and delta chain and immunoglobulin heavy and light chain ge...

journal_title：Leukemia research

authors： Akiyoshi TT,Kimura N,Uike N,Kozuru M,Tamura K,Takihara Y,Hisano S,Nishimura J,Kikuchi M

更新日期：1991-01-01 00:00:00

abstract：:We describe the properties of three monoclonal antibodies (McAbs) (21H73, 37G7 and 49C12) against K562 cell surface antigens correlated with differentiation induced by 12-O-tetradecanoyl phorbol-13-acetate (TPA). Each of the McAbs immunoprecipitated K562 cell surface antigen with molecular weight (MW) of approximately...

journal_title：Leukemia research

authors： Kubota Y,Tanaka T,Irino S

更新日期：1991-01-01 00:00:00

abstract：:IL-3 stimulated the production of IL-6 from a bone marrow-adherent cell population, macrophages and from hemopoietic supportive stromal cell lines. It also induced IL-6 production from a stem cell-enriched population of bone marrow cells which did not produce IL-6 without stimulation. In contrast, stimulation with IL-...

journal_title：Leukemia research

authors： Miyashita M,Sugimoto K,Suzuki J,Taniguchi S,Aramaki K,Mori KJ

更新日期：1991-01-01 00:00:00

abstract：:The prognosis of patients with advanced refractory lymphoma remains poor. We have carried out a Phase II study of continuous high dose intravenous recombinant interleukin 2 alone without LAK cells in this group of patients. Eight patients have so far been treated, 4 with non-Hodgkins lymphoma and 4 with Hodgkins disea...

journal_title：Leukemia research

authors： Lim SH,Worman CP,Callaghan T,Jewell A,Smith MP,Goldstone AH

更新日期：1991-01-01 00:00:00

abstract：:Medullary dyserythropoiesis with reduced production of erythrocytes is an early and consistent feature of myelodysplastic syndromes (MDS). The mechanism underlying the disturbed red cell proliferation and maturation is presently unknown. In order to study the role of erythropoietic growth factors, we determined by rad...

journal_title：Leukemia research

authors： Aul C,Arning M,Runde V,Schneider W

更新日期：1991-01-01 00:00:00

abstract：:The basis of intrinsic resistance of blasts from patients with acute non-lymphocytic leukemia (ANLL) to methotrexate was studied. MTX polyglutamate formation was measured in blast cells from 19 patients with ANLL and in 7 pediatric patients with acute lymphocytic leukemia (ALL), after in vitro incubation for 24 h with...

journal_title：Leukemia research

authors： Lin JT,Tong WP,Trippett TM,Niedzwiecki D,Tao Y,Tan C,Steinherz P,Schweitzer BI,Bertino JR

更新日期：1991-01-01 00:00:00

abstract：:Changes of phosphoprotein patterns in HL-60 cells were studied during short exposures to 1 alpha, 25-dihydroxyvitamin D3 [1,25(OH)2D3]. One hundred nanometers 1,25(OH)2D3 dephosphorylated at least three proteins in 6 h: phosphoproteins with molecular weights of 82 kD (pp82), 33 kD (pp33), and 31 kD (pp31). Phosphoryla...

journal_title：Leukemia research

authors： Ueda T,Sawada H,Okuda T,Yumoto Y,Kato Y,Ogawa K,Tashima M,Yoshida Y,Okuma M

更新日期：1991-01-01 00:00:00

abstract：:Forty-three patients were studied to determine whether light chain gene rearrangements may occur in hematopoietic cells not pertaining to the B-lineage. In only one patient, affected by T-cell lymphoblastic lymphoma, one kappa light chain allele was rearranged. Neither at the protein level nor at the RNA level the rea...

journal_title：Leukemia research

authors： Moretti L,Mariano MT,Donelli A,Montagnani G,Sarti M,Grantini M,Di Prisco U,Torelli U,Narni F

更新日期：1991-01-01 00:00:00

abstract：:The role of hemin induction of K562 in inositol phospholipid metabolism has not been previously studied. K562 cells were induced to synthesize hemoglobin upon addition of bovine hemin to the culture media. The phospholipid content of K562 was determined before and after the addition of hemin. The results of this study...

journal_title：Leukemia research

authors： Klette KL,Wanda PE

更新日期：1991-01-01 00:00:00

abstract：:We determined the position of the breakpoint within the bcr gene in 22 patients with Philadelphia-positive chronic myelocytic leukemia using conventional Southern-blots and analyzed its relationship to thrombopoiesis. After DNA digestion with restriction endonucleases (Hind III, Bam HI and Bgl II), we localized the br...

journal_title：Leukemia research

authors： Inokuchi K,Futaki M,Yamada T,Tanabe Y,Dan K,Shinohara T,Kuriya S,Nomura T

更新日期：1991-01-01 00:00:00

abstract：:This study examines the occurrence, subtype and possible significance of haemopoietic inhibitory T cells (HIT cells) in patients with myelodysplasia (n = 21), acute myeloblastic leukaemia (n = 8) and in normal subjects (n = 13) using an autostimulary CFU-GM stem cell assay. HIT cells were detected when colony numbers ...

journal_title：Leukemia research

authors： Smith MA,Smith JG

更新日期：1991-01-01 00:00:00

abstract：:Bone marrow cells from 109 patients (median age 60) with newly diagnosed acute myeloid leukaemia (AML) were prospectively immunophenotyped (IP) and the prognostic value of monoclonal antibody (MAB) reactivities was analysed to detect differences in complete remission rates and survival, not only between groups of MAB ...

journal_title：Leukemia research

authors： Kristensen JS,Hokland P

更新日期：1991-01-01 00:00:00

abstract：:A total of 854 patients with anti-HTLV-I antibody positive ATL, newly diagnosed from 1983 to 1987, were analyzed for prognostic factors. There were 466 males and 388 females with a mean age of 57.1. Of these 269 (31.5%) were alive with a median follow-up time of 14 months from diagnosis, while 585 (65.5%) were dead wi...

journal_title：Leukemia research

authors：

更新日期：1991-01-01 00:00:00

abstract：:The ability of A-MuLV to transform bone marrow cells on in vitro culture in agarose is enhanced by inclusion of conditioned media during infection and culture. The conditioned medium of a non-virus producing A-MuLV transformed fibroblast cell line was synergistic with medium from Whitlock-Witte long-term bone marrow c...

journal_title：Leukemia research

authors： Palumbo GJ,Ozanne B,Kettman JR

更新日期：1991-01-01 00:00:00

abstract：:Chemosensitivity of purified AML blasts to daunomycin was examined under conditions of CSF stimulation and compared with clinical outcome of DNR combination chemotherapy. AML blasts from 16 patients were purified, incubated serum-free in the presence of optimal concentrations of a complete cocktail of IL-3, GM-CSF and...

journal_title：Leukemia research

authors： Santini V,Nooter K,Delwel R,Löwenberg B

更新日期：1990-01-01 00:00:00

abstract：:3-Aminobenzamide, a general inhibitor of poly(ADP-ribose)polymerase, potentiated the triamcinolone acetonide-mediated growth inhibition and lysis of the glucocorticoid-sensitive CEM-C7 human leukemic cell line. This potentiation was dose-dependent with maximal response being detected at 3 mM 3-aminobenzamide, and was ...

journal_title：Leukemia research

authors： Meyer AS,Schlechte JA,Schmidt TJ

更新日期：1990-01-01 00:00:00

abstract：:Recombinant TNF was capable of inducing differentiation of human leukemic ML-1 cells in the monocytic pathway. Recombinant interleukin 6 did not have the activity but it could significantly increase the activity of recombinant TNF. Both of these molecules were found to play a similar role in PWM-induced conditioned me...

journal_title：Leukemia research

authors： Samal B,Stearns G,Crandall C,Arakawa T,Boone T

更新日期：1990-01-01 00:00:00

abstract：:Proto-oncogene transcriptional activation was analyzed in a group of MCF 247 MuLv-induced T-cell lymphomas to identify transformation-specific gene activations and determine whether the proviral insertion near a myc gene could promote a peculiar mechanism of transformation through a differential proto-oncogene express...

journal_title：Leukemia research

authors： Boiocchi M,Dolcetti R,Maestro R,Feriotto G,Rizzo S,De Re V,Sonego F

更新日期：1990-01-01 00:00:00

abstract：:Accumulation of cytosine arabinoside triphosphate (araCTP) from a range of cytosine arabinoside (araC) concentrations (1-50 microM) was measured during incubations of leukemic cells freshly isolated from patients with acute leukemia. In all but one patient, increments in extracellular araC above 10 microM did not incr...

journal_title：Leukemia research

authors： Jamieson GP,Snook MB,Wiley JS

更新日期：1990-01-01 00:00:00

abstract：:The remarkable capacity of the bone marrow to compensate for blood loss and for reduced atmospheric oxygen tension has been found to be mediated by a renal hormone, named erythropoietin. It is produced by peritubular interstitial cells in response to renal hypoxia, but molecular engineering has permitted large scale p...

journal_title：Leukemia research

authors： Erslev AJ

更新日期：1990-01-01 00:00:00

abstract：:Fractionation of mononuclear cells from human fetal liver provides a cell population at early stages of myeloid differentiation which, when cultured, generates neutrophils and macrophages for up to a month. These studies describe the further purification of an undifferentiated myeloid blast cell population by rosette ...

journal_title：Leukemia research

authors： Bunce CM,Patton WN,Pound JD,Lord JM,Brown G

更新日期：1990-01-01 00:00:00

abstract：:We studied differentiation inducing effects of retinoic acid (RA), 1 alpha, 25-dihydroxyvitamin D3 (D3) and interferons (IFNs), alone and in combination, on fresh myeloid leukemic cells from 8 patients. RA not only induced the differentiation of leukemic cells in 5/8 cases, but potentiated differentiation by IFNs eith...

journal_title：Leukemia research

authors： Nakamaki T,Sakashita A,Hino K,Suzuki K,Tomoyasu S,Tsuruoka N,Honma Y,Hozumi M

更新日期：1990-01-01 00:00:00

abstract：:Hodgkin and Sternberg Reed (H and SR) cells, the putative malignant cells of Hodgkin's disease carry regularly T-cell activation antigens, like CD30 and CD25 (low affinity IL-2 receptor). We have investigated the Hodgkin cell line L540, bearing characteristic markers of H and SR cells for its expression of the low aff...

journal_title：Leukemia research

authors： Tesch H,Herrmann T,Abts H,Diamantstein T,Diehl V

更新日期：1990-01-01 00:00:00

abstract：:In this study we investigated the Ara-CTP-forming capacity of leukemic cells in different phases of the cell cycle. Cells from two leukemic cell lines and leukemic bone marrow cells from patients and rats (BNML model) with acute myelocytic leukemia were separated according to cell cycle phase by means of an albumin de...

journal_title：Leukemia research

authors： Richel DJ,Colly LP,Arentsen-Honders MW,Starrenburg CW,Willemze R

更新日期：1990-01-01 00:00:00

abstract：:5-Aza-2'-deoxycytidine (5-AZA-CdR), a potent inhibitor of DNA methylation and an active antileukemic agent, produced an induction of cytidine deaminase activity in human HL-60 myeloid leukemic cells. This increase in enzyme activity correlated with the induction of differentiation of the HL-60 leukemic cells as shown ...

journal_title：Leukemia research

authors： Momparler RL,Laliberté J

更新日期：1990-01-01 00:00:00

abstract：:Argininosuccinate synthetase (ASS) is a ATP-dependent and rate-limiting enzyme of the urea cycle which catalyzes L-citrulline to L-arginine in combination with argininosuccinate lyase (ASL). We demonstrate here that (a) human normal T and B lymphocytes did not express ASS activity, (b) however, three adult T leukemia ...

journal_title：Leukemia research

authors： Sugimura K,Kimura T,Arakawa H,Ohno T,Wada Y,Kimura Y,Saheki T,Azuma I

更新日期：1990-01-01 00:00:00

abstract：:Thirteen hairy-cell leukaemia patients were treated with IFN-beta (6 X 10(6) IU/m2) for 7 days, alternate weeks, for three cycles. IFN-beta was then continued at the same dose twice a week for 24 weeks. Treatment was discontinued in 2 non-responders and 2 partial responders (1 haem PR, 1 path PR) because of complicati...

journal_title：Leukemia research

authors： Liberati AM,Fizzotti M,Di Clemente F,Senatore M,Berruto P,Falini B,Martelli MF,Grignani F

更新日期：1990-01-01 00:00:00

abstract：:The K562 leukemia cell line has been extensively used in studies of erythroid differentiation but it has been less well appreciated that treatment of K562 cells with the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA) leads to loss of their erythroid properties and to acquisition of several megakaryoblastoi...

journal_title：Leukemia research

authors： Alitalo R

更新日期：1990-01-01 00:00:00

abstract：:CFW/D mice injected neonatally with a type B retrovirus, (DMBA-LV), rapidly develop thymic lymphomas. The present study examined simultaneously the karyotype and the expression of surface antigens on thymocytes from DMBA-LV treated mice at 28, 35 and 42 days of age during the development of lymphomas, and of cells fro...

journal_title：Leukemia research

authors： Mueller RE,Ball JK,Chan FP

更新日期：1989-01-01 00:00:00

abstract：:This study examined the immunoregulatory role of recombinant interleukin 4 (IL-4), also known as B-cell stimulating factor 1, on the generation of cytotoxic effector cells from normal and leukaemic human blood mononuclear cells. When tested on cells from normal individuals, the addition of IL-4 to mixed lymphocyte cul...

journal_title：Leukemia research

authors： Jin BQ,Lopez AF,Gillis S,Juttner CA,Vadas MA,Burns GF

更新日期：1989-01-01 00:00:00